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PIXELLS

PiXELLs are condensates that dissociate upon blue light stimulation. The basic module consists of the algal-derived Pix proteins. PixD forms dimers and PixE is a monomer. When expressed together they associate in large complexes of a 10:5 or 10:4 ratio. Fusing these nucleating proteins to sticky IDRs drives their condensation.

Other optogenetic condensates system that phase separates upon blue light exposure is called optoDroplets, Corelets and CasDrop.

PROS:
+ common molecular grammar of IDR
+ IDR subunit is highly tunable
+ optogenetic control

CONS:
- large constructs
- potential interference with endogenous processes depending on IDR
- prolonged blue light exposure is toxic in mammalian cell models
- two-component system

PiXELLs sequences:

currently unavailable