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CÖRELET

Corelets are optogenetic inducible condensates. The module consists of a ferritin core that is fused to an optogenetic interaction domain that can recruit sticky IDRs that drive subsequent phase separation.

Other optogenetic condensate systems are optoDroplets and PixELLs.

PROS:
+ common molecular grammar of IDR
+ IDR subunit is highly tunable
+ optogenetic induction

CONS:
- large constructs
- potential interference with endogenous processes depending on IDR
- prolonged blue light exposure is toxic in mammalian cell models
- two-component system

Optodroplet sequences:

Optodroplet sequences and constructs can be found at Addgene.